Figure 1.

Identification and complementation of ddl alleles. A, Ecotype Wassilewskija of Arabidopsis (WS), ddl-1 complemented with genomic DDL, and the ddl-1 mutant. B, DDL gene and protein structure. ddl-1 and ddl-2 are T-DNA insertion alleles and ddl-3 is a TILLING point mutation. Three nuclear localization signals are predicted at the N terminus of the protein. The FHA domain stretches from residues 218 to 282 at the C terminus of the protein. C, RT-PCR results show that DDL RNA cannot be amplified in ddl-1 or ddl-2 homozygous mutants. Labels above gels indicate RNA source. Lines below indicate primers used. CLV1 primers were used as RNA quality controls.