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ARF GTPase-activating activity assay of RPA protein. A, Autoradiograph showing GTP binding to proteins as indicated. Note strong GTP-binding activity of ARF1 and U5; very weak binding for ARF3. B, GTPase-activating activity of RPA on ARF1 GTPase assessed by hydrolysis of α-32P-GTP-ARF1. RPA, No ARF1 protein added; GTP, no protein added. C, GTPase-activating activity of RPA on U5 protein assessed by hydrolysis of GTP bound to U5. RPA, No U5 protein added; GTP, no protein added.
