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. 2006 Jul;141(3):966–976. doi: 10.1104/pp.106.077818

Figure 6.

Figure 6.

Functional complementation of glo3Δ gcs1Δ mutant in yeast by RPA. A, glo3Δ gcs1Δ mutant cell transformed with RPA and the GCS1 positive control could grow at 37°C, whereas the cells transformed with GCS112 (12 amino acids) could not grow. The PPY147.28.2A cells containing a temperature-sensitive GCS1 allele also could not grow at 37°C. B, All yeast cells could grow at the permissive temperature 23°C. C, Schematic drawing depicts the position of yeast cells transformed with genes as indicated. RPA, RPA gene; RPArecovery, RPA plasmid was reisolated from single yeast colony and retransformed into the mutant; GCS1, GCS1 gene; GCS112, GCS1 fragment containing 12 amino acids from the N terminus; PPY147.28.2A, control cells not transformed.