Table I.
List of primers
| Construct | Primer | Sequence |
|---|---|---|
| Pro:RPA-GUS:Ter construct | 3118P-5′ | 5′-GTAAGCTTCTTTTAGTTTTATTCTAGTCG-3′ |
| 3118P-3′ | 5′-CTGGATCCTGTAGGGATGTGAAACAATAT-3′ | |
| 3118G-5′ | 5′-GTCGGATCCATGGCGTCTGAGAATCTGAATG-3′ | |
| 3118G-3′ | 5′-GCAGGATCCCAAACCCATAAGCTGGAAG-3′ | |
| 3118T-5′ | 5′-GTCGAGCTCTCTGTTGCTTCCAGCTTATG-3′ | |
| 3118T-3′ | 5′-AGAGAGCTCAAATCTCAAATGACAACC-3′ | |
| RPA-EGFP construct | RPA-BamHI-5′ | 5′-GTCGGATCCATGGCGTCTGAGAATCTGAATG-3′ |
| RPA-KpnI-3′ | 5′-GCAGGTACCCAAACCCATAAGCTGGAAG-3′ | |
| RPA-128AA-KpnI-3′ | 5′-CTTGGTACCTCCTCAGCCTTGCTTTTTGC-3′ | |
| RPA-246AA-KpnI-3′ | 5′-CTTGGTACCCTGTTTTGAACATTGTCCGC-3′ | |
| RPA-316AA-KpnI-3′ | 5′-GTTGGTACCTTTCCAAAGTACTGAGCAGAGG-3′ | |
| RPA-79AA-NcoI-5′ | 5′-CAACCATGGACAACAACTCTGCTGATC-3′ | |
| RPA-79AA-NcoI-3′ | 5′-CAACCATGGAACCCATAAGCTGGAAGC-3′ | |
| His-RPA construct | RPA-EcoRI-5′ | 5′-GTGAATTCCATGGCGTCTGAGAATCTGAATG-3′ |
| RPA-XhoI-3′ | 5′-GTACTCGAGCCAAACCCATAAGCTGGAAGCA-3′ | |
| His-ARF construct | ARF1-BamHI-5′ | 5′-GAGGATCCATGCGTATTCTGATGGTTGGT-3′ |
| ARF1-XhoI-3′ | 5′-GATCTCGAGCTAAGCCTTGTTTGCGATGT-3′ | |
| U5-BamHI-5′ | 5′-GAGGACCATGAGAATTCTGATGGTTGGT-3′ | |
| U5-XhoI-3′ | 5′-GATCTCGAGCAGCAAATCAACTCCACCACG-3′ | |
| ARF3-BamHI-5′ | 5′-GAGGATCCGCTCGAATCCTCGTCCTCGGT-3′ | |
| ARF3-NotI-3′ | 5′-GAGCGGCCGCTTAGCCACTTCCCGACTTCA-3′ | |
| U2-BamHI-5′ | 5′-GAGGATCCATGCGTATCCTCATGGTGGGT-3′ | |
| U2-XhoI-3′ | 5′-GATCTCGAG CTAAGCCTTGTTTGCGATGT-3′ | |
| PGCS1-RPA construct | EcoRI-Gcs1-5′ | 5′-CTCGAATTCAGAAACATAAGATGGAATCC-3′ |
| Gcs1 Lig RPA | 5′-ACCCGCAGGCGTAAACTCAAAG-3′ | |
| RPA-BspE1-3′ | 5′-ATCTCCGGA TCAAACCCATAAGCTGGAAG-3′ |