Figure 3.
Effect on gene expression patterns of mutations affecting PSII, PC, PSI, and of inhibitors that block electron transfer from PSII to the cytb6/f complex in wild-type cells. All cultures were incubated in the dark for 20 h (D). Cultures of the 4A+ strain and the four mutants were then exposed to white light for one (L1) and 2 h (L2). For inhibitor studies, dinoterb (final concentration 30 μm), DCMU (final concentration 3 μm), and DBMIB (final concentration 1.5 μm) were added to cultures 40 min prior to shift to light. Samples were processed as described in the legend of Figure 2. Note that DBMIB caused overaccumulation of HSP70B mRNA in the dark and in the light, an observation that was not pursued.