Table II.
Analysis of progeny from crosses of mutants with wild type for cosegregation of the mutant phenotypes
ac−, Acetate requiring; LS, no growth at 80 μE m−2 s−1; HLS, no growth at 500 μE m−2 s−1; LR, light resistant; lrc, light regulation of chlorophyll biosynthesis gene expression.
| Crosses Performed | Phenotypes of Progeny Assayed on Plates | Regulatory Phenotypes of Eight Clonesa |
|---|---|---|
| ΔpetA (mt+) × 4A− (mt−) | 152ac−, LS/12ac+, LR | 4lrc−, ac−, mt−/4lrc+, ac+, mt+a |
| petD-PWYE (mt+) × 4A− (mt−) | 94ac−, LS/6ac+, LR | 4lrc−, ac−, mt−/4lrc+, ac+, mt+a |
| mcd1-F16 (mt−) × 4A+ (mt+) | 23ac−, LS/24ac+, LR | 4lrc−, ac−, LS/4lrc+, ac+, LR |
| tca1-693 (mt−) × 4A+ (mt+) | 21ac−, LS/27ac+, LR | 4lrc−, ac−, LS/4lrc+, ac+, LR |
| P1-15 (mt+) × 4A− (mt−) | 24ac−, HLS/23ac+, LR | 4lrc−, ac−, HLS/4lrc+, ac+, LR |
| P2-26 (mt+) × 4A− (mt−) | 20ac−, LS/27ac+, LR | 4lrc−, ac−, LS/4lrc+, ac+, LR |
To ensure the analysis of recombinants (by RNA-blot techniques) from these crosses, the lrc phenotype was assayed only in ac− progeny that was mt− and in ac+ progeny that was mt+.