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Effect of GYPGQV-NH2, a PAR-4 agonist peptide and its reverse peptide VQGPYG-NH2 on release of IL-8 from A549 cells. Cells were incubated with various concentrations of GYPGQV-NH2 or VQGPYG-NH2 at 37°C for 16 h (A), or with 10 μM of GYPGQV-NH2 for 2 h, 8 h and 16 h (B). Values shown are mean ± SE for 5 separate experiments. *P < 0.05 compared with the response to medium alone control; †P < 0.05 compared with the response to VQGPYG-NH2 at the same concentration.
