Table 2.

Effect of hirudin on IL-8 release from A549 cells induced by thrombin

Compound (U/ml)	IL-8 released (pg/ml)
	2 h	16 h
Medium alone	18.7 ± 1.6	64.8 ± 7.2
Thrombin 3	40.1 ± 11.1	181 ± 32
Hirudin 3	18.4 ± 1.7	52.8 ± 5.7
Thrombin 3 + Hirudin 3	21.9 ± 7.2*	73.9 ± 12.9*
Thrombin 10	65.3 ± 18.4	478 ± 131
Hirudin 10	18.9 ± 2.1	59.3 ± 12.6
Thrombin 10 + Hirudin 10	26.8 ± 6.7*	119 ± 16.6*

Data shown are mean ± SE for five separate experiments. Thrombin, hirudin, and thrombin with hirudin were added to cells for 2 h or 16 h. *P < 0.05 compared with the corresponding concentration of thrombin alone.