Table 4.

Effect of the inhibitors of tryptase or elastase on tryptase or elastase induced release of IL-8 from A549 cells

Compound (μg/ml)	IL-8 released (pg/ml)
Medium alone	24.6 ± 1.5
Tryptase 2.0	105.2 ± 5.3*
Elastase 0.3	108.4 ± 13.2*
Benzamidine 30	37.6 ± 4.4
Leupeptine 30	27.4 ± 5.6
MSACK 30	22.1 ± 4.3
Tryptase 2.0 + Benzamidine 30	66.9 ± 4.8†
Tryptase 2.0 + Leupeptin 30	100 ± 1.7
Elastase 0.3 + MSACK 30	26.9 ± 3.6†

Data shown are mean ± SE for five to six separate experiments. Stimulus, inhibitor, or stimulus with inhibitor was incubated with cells for 16 h. * P < 0.05 compared with the response to medium alone; ^†P < 0.05 compared with the corresponding stimulus alone.