FIG. 1.

Schematic representation of vanD gene clusters and of recombinant plasmids. Open arrows represent coding sequences and indicate the direction of transcription. The double-headed arrow indicates the 2,368-bp PCR product previously sequenced (23). The PCR fragment internal to the vanD gene of strain 10/96A used as a probe in hybridization experiments is indicated above the corresponding region. Vertical dashed lines indicate separation of the vanS_D gene of 10/96A into two parts S_D′ and vanS_D′. The vertical bar in the vanY_D gene of 10/96A indicates the position of the frameshift mutation leading to a predicted truncated protein. The inserts in recombinant plasmids are represented by solid lines, and the vectors are indicated in parentheses. Arrowheads represent binding sites and orientation of oligodeoxynucleotides used for amplification.