TABLE 2.
MICs of glycopeptides and cytoplasmic peptidoglycan precursors synthesized by VanD-type strains
| E. faecium strain | MIC (μg/ml)a
|
% of peptidoglycan precursorsb
|
||||
|---|---|---|---|---|---|---|
| VM | TE | UDP-MurNAc-tripeptide | UDP-MurNAc-tetrapeptide | UDP-MurNAc-pentapeptide | UDP-MurNAc-pentadepsipeptide | |
| BM4339 | 64 | 4 | 19 | 21 | 2 | 58 |
| BM4416 | 128 | 64 | 7 | 24 | <1 | 69 |
| 10/96A | 256 | 4 | 0 | 2 | 3 | 95 |
a
MICs were determined by the method of Steers et al. (52). Abbreviations: VM, vancomycin; TE, teicoplanin.
b
The bacteria were grown without vancomycin to the mid-exponential phase, and peptidoglycan synthesis was inhibited by addition of ramoplanin to the cultures for 15 min.