TABLE 3.
d,d-Peptidase activities in extracts from E. faecium 10/96Ac
| Concn of VMa (μg/ml) | Mean d,d-dipeptidase activityb ± SD (nmol min−1 mg−1) | Mean d,d-carboxypeptidase activityd ± SD (nmol min−1 mg−1) in fraction
|
|
|---|---|---|---|
| Membrane | Cytoplasmic | ||
| 0 | 1.6 ± 0.5 | 24 ± 5.9 | 3.9 ± 0.3 |
| 1 | 2.4 ± 0.6 | 23 ± 6.5 | 5.2 ± 0.6 |
| 8 | 2.3 ± 0.6 | 26 ± 5.3 | 3.9 ± 1.2 |
| 64 | 1.3 ± 0.5 | 37 ± 6.8 | 3.8 ± 0.8 |
a
VM, vancomycin.
b
The activity was measured in the supernatant of lysed bacteria after centrifugation at 100,000 × g.
c
Results are obtained from a minimum of three independent extracts.
d
The activities were measured in the supernatant and in the resuspended pellet fraction after centrifugation of lysed bacteria at 100,000 × g for 45 min.