TABLE 4.
d,d-Peptidase activities in extracts from E. faecalis JH2-2 derivativesb
| Strain/plasmid | van gene (strain) | Mean d,d-dipeptidase activitya (nmol min−1 mg−1) | Mean d,d-carboxypeptidase activityc ± SD (nmol min−1 mg−1) in fraction
|
|
|---|---|---|---|---|
| Membrane | Cytoplasmic | |||
| JH2-2 | None | NDd | ND | ND |
| JH2-2/pAT635 | vanXD (BM4339) | 1.3 ± 0.5 | NAe | NA |
| JH2-2/pAT636 | vanXD (10/96A) | 0.8 ± 0.1 | NA | NA |
| JH2-2/pAT633 | vanYD (BM4339) | NA | 6.4 ± 1.5 | 1.9 ± 0.5 |
| JH2-2/pAT634 | vanYD (10/96A) | NA | ND | ND |
a
The activity was measured in the supernatant of lysed bacteria after centrifugation at 100,000 × g.
b
Results are obtained from a minimum of three independent extracts.
c
The activities were measured in the supernatant and in the resuspended pellet fraction after centrifugation of lysed bacteria at 100,000 × g for 45 min.
d
ND, not detectable.
e
NA, not applicable.