FIG. 2.

Antiviral effect of NHC on cpBVDV. EC₉₀s mentioned in the text are deduced from the graphs. Error bars for all data points are included but are very often less than the size of the symbol. (A) Dose-dependent viral RNA yield reduction in MDBK cell supernatant. Cell supernatant fluids were harvested 24 h (on exponentially growing cells) or 72 h (on confluent monolayer cells) postinfection, and viral RNA levels were quantified by Q-RT-PCR. The zero value corresponds to no drug added to the medium. ▿, ribavirin (confluent cells); •, ribavirin (exponentially growing cells); ▪, NHC (confluent cells); ○, NHC (exponentially growing cells); ▾, control compound showing no activity. VL, viral load; S.D., standard deviation. (B) Dose-dependent reduction of viral RNA in a yield reduction assay. cpBVDV was recovered from a single-cycle infection assay, and the virus yield was titrated by means of a plaque assay. •, NHC; ▪, NN-DNJ (47).