FIG. 6.
Characteristic HPLC chromatographic profiles of M. smegmatis intracellular amino acid pools. Intracellular amino acid pools of M. smegmatis mc2155 untreated (A) and treated with DCS (B) were prepared and analyzed by a reverse-phase HPLC method as described in Materials and Methods. The synthetic amino acid l-homo-arginine (l-hArg) served as an internal standard for quantifying the abundance of each individual amino acid. Peaks for various amino acids are indicated, with l- and d-alanine highlighted.