TABLE 1.
P. aeruginosa strains and plasmids used in this study
| P. aeruginosa strain or plasmid | Description | Source or reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Prototroph | 10 |
| HN1112 | Spontaneous streptomycin-resistant (rpsL) derivative of PAO1 | This study |
| HN1113 | HN1112 ΔemrE | This study |
| K1119 | PAO1 ΔmexAB-oprM | 9 |
| HN1114 | Spontaneous streptomycin-resistant (rpsL) derivative of K1119 | This study |
| HN1115 | HN1113 ΔmexAB-oprM | This study |
| K1589 | Spontaneous streptomycin-resistant (rpsL) derivative of PAO1 made ΔmexR ΔmexB | K. Poole |
| HN1116 | K1589 ΔemrE | This study |
| Plasmids | ||
| pEX18Tc | Broad-host-range gene replacement vector; sacB, tetracycline resistant | 4 |
| pXZL1307 | pEX18Tc::ΔemrE | This study |
| pELCT04 | pK18mob sacB::ΩHgr and ΔmexAB-oprM, kanamycin and HgCl2 resistant | 9 |
| pBluescript II SK(+) | Cloning vector, ampicillin resistant | Stratagene |
| pXZL1582 | pBluescript II SK(+)::emrEPae | This study |