Figure 5.

(A) Flow cytometric analysis of the surface expression of GPI-anchored ALCAM on K562-GPI-ALCAM using J4-81. The shaded histogram represents the isotype-matched control, and the open histogram represents J4-81 staining. Depicted is the fluorescence intensity versus the cell count. (B) GPI-anchored ALCAM-mediated adhesion cannot be induced by disruption of the actin cytoskeleton. K562-GPI-ALCAM cells (white bars) and K562-ALCAM cells (black bars), untreated or pretreated with CytD (2.5 μg/ml) or LatA (5 μg/ml), were allowed to adhere to immobilized ALCAM-Fc (250 ng/ml) for 45 min at 37°C, and ALCAM-specific adhesion was determined. Specific adhesion is expressed as the mean percentage ± SD of adherent cells from triplicate wells. Data are representative of four experiments.