TABLE 1.
Candidacidal activity of noncandidacidal concentrations of hLF(1-11) with or without fluconazolea
| Peptide (concn) | No. of surviving C. albicans cellsb with fluconazole concn (μg/ml) of:
|
|||
|---|---|---|---|---|
| 200 | 150 | 100 | 0 | |
| hLF(1-11) (8 μM) | (14 ± 10) × 103c,d | (1.4 ± 2) × 104c,d | (1.1 ± 1) × 105 | (3.6 ± 3) × 105 |
| hLF(1-11) (4 μM) | (3.4 ± 3) × 104c,d | (3.4 ± 3) × 104c,d | (31 ± 4) × 104c | (7.9 ± 2) × 105 |
| hLF(4-11) (8 μM) | (5.9 ± 2) × 105 | (1.4 ± 1) × 106 | (14 ± 10) × 105 | (12 ± 2) × 105 |
| None | (2.4 ± 3) × 105 | (4.3 ± 3) × 105 | (5.6 ± 3) × 105 | (1 ± 1) × 106 |
a
106 CFU of fluconazole-resistant C. albicans/ml were incubated with combinations of hLF(1-11) and fluconazole for 2 h at 37°C, and then the number of surviving cells was determined microbiologically. The peptide hLF(4-11) served as the negative control.
b
Data are means ± SD from at least three independent experiments.
c
Significantly different (P < 0.05) from values obtained with C. albicans exposed to hLF(1-11) alone.
d
Significantly different (P < 0.05) from values obtained with C. albicans exposed to fluconazole alone.