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. 2005 Jan;7(1):57–66. doi: 10.1593/neo.04406

Figure 4.

Figure 4

Immunofluorescence labeling of malignant keratinocytes and vessels in transplants grafted in nude (A) or Rag-1-/- (B) mice. (A) HaCaT II-4 (a and d), HaCaT A5-RT3 (b and e), and Caski (c and f) cells were implanted for 3 weeks into WT (a–c) and PAI-1-/- (d–f) nude mice. (B) HaCaT II-4 (a and c) and HaCaT A5-RT3 (b and d) cells were grafted for 3 weeks into WT (a and b) and PAI-1-/- (c and d) Rag-1-/- mice. Malignant cells were detected on cryostat sections by using anticytokeratin Ab (green), and vessels were detected using an anticollagen type IV Ab (red). At all times after grafting, collagen type IV labelings were codistributed with endothelial cells recognized by antimouse platelet endothelial cell adhesion molecule (PECAM) immunostaining (data not shown). C = carcinoma cells; G = collagen gel; H = host connective tissue. Original magnification, x 100.