Figure 5.

Semiquantitative analysis of angiogenesis of tumors transplanted into nude or Rag-1^-/- mice. HaCaT II-4 (a and d), HaCaT A5-RT3 (b and e), and CasKi (c) tumor cells were grafted for 3 weeks into PAI-1^-/- (white bars) and WT (black bars) mice in a nude (a–c) or Rag-1^-/- (d and e) background. Morphometric assessment of angiogenesis was scored as follows: vessels undetected in the collagen gel: score (-); blood vessels infiltrating the collagen gel and getting in close apposition to the epithelial layer: score (++); and blood vessels intermingling with invasive epithelial tumor sprouts: score (+++). n = number of animals per group. Chi-square analysis was used; P is indicated.