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Activation of a G2 checkpoint reduces ABHA-induced cell death. (A) HeLa cells were synchronized by hydroxyurea block release as in Figure 5, treated with ABHA (ABHA), ICRF193 (ICRF), or nocodazole (Noco.), or added in combination as indicated, 2 h after release, and then harvested at 24 h. (B) Proportion of subdiploid cells at 24 h from five independent experiments similar to those in A, with no addition (HU), ABHA only, ICRF193 only, or coaddition of ABHA and ICRF193.
