Figure 4.

cR Light–Induced Nuclear Localization and Degradation of PHYA.

The subcellular localization of PHYA in wild-type pea hook cells exposed to cR light was visualized by immunofluorescence of FITC using monoclonal antibody mAA01. (A), (D), (G), (J), (M), and (P) show epifluorescence images of FITC; the nuclei in (A), (D), (G), (J), (M), and (P) were visualized with epifluorescence images of H33258 in (B), (E), (H), (K), (N), and (Q), respectively. The positions of these respective cells are evident from Nomarski differential interference contrast images (C), (F), (I), (L), (O), and (R).

(A) to (C) After 1 min under cR illumination.

(D) to (F) After 5 min under cR illumination.

(G) to (I) After 10 min under cR illumination.

(J) to (L) After 0.5 hr under cR illumination.

(M) to (O) After 4.5 hr under cR illumination.

(P) to (R) After 6 hr under cR illumination.

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