Figure 1.

Restoration of peroxisomes in CHO mutant cells. (a and f) Peroxisome-deficient ZPG208 cells; (b), peroxisome-restored ZPG208 after lipofection with a combined pool (C8) of rat cDNA library; (c) ZPG208 transfected with pBKCMV·RnPEX3 plasmid; d, e, and g) 208P3 cells, stable RnPEX3-transformants of ZPG208 cells; (h) ZPG209 was transfected with pCMVSPORT·HsPEX3. (a–c) Fluorescent micrograph of GFP-expressing ZPG208. Cells were stained with antisera to catalase (d and h), PTS1 (e), and PMP70 (f and g), respectively. Magnification, 630×; bar, 20 μm.