Figure 4.

Mutation of PEX3 in ZPG208 cells. (A) Mutation analysis of PEX3 in ZPG208. Partial sequence and deduced amino acid sequence of PEX3 cDNA isolated from CHO-K1 (left) and a mutant ZPG208 (right) are shown. A point mutation at nucleotide residue 413 changes a codon (GGA) for Gly¹³⁸ (Figure 2, solid arrowhead) to a codon (GAA) for Glu¹³⁸. (B) Transfection of mutated Chinese hamster PEX3. ZPG208 cells were transfected with Chinese hamster PEX3, ClPEX3 (a), or ZPG208-derived PEX3, ClPEX3G138E (b). A fluorescence micrograph of intracellular localization of PTS2-GFP is shown. Magnification, 630×; bar 20 μm.