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. 2000 Jun;11(6):2085–2102. doi: 10.1091/mbc.11.6.2085

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Copyright © 2000, The American Society for Cell Biology

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Intracellular localization of Pex3p. (A) Morphological analysis. Flag-HsPEX3 was expressed in CHO-K1 cells. (a and b) CHO-K1 cells stained with antibodies to flag and catalase, respectively; (c and d) PEX2-impaired, peroxisome remnant-positive Z65 was transfected with flag-RnPEX3-HA and stained with antibodies to HA and PMP70, respectively. Magnification, 630×; bar, 20 μm. (B) Intraperoxisomal localization of Pex3p. Immunoblotting was done with antibodies specific for Pex3p, Pex12p, PMP22, and catalase. Lane 1, rat liver peroxisomes (10 μg); lanes 2 and 3, soluble and membrane fractions after the sodium carbonate treatment (Fujiki et al., 1982b) of peroxisomes (25 μg); lanes 4 and 5, soluble and membrane fractions after the Triton X-114 treatment (Bodier, 1981) of peroxisomes (25 μg).