Figure 9.

Functional and topogenic regions of Pex3p. N-terminally flag-tagged and C-terminally EGFP-fused rat Pex3p and its variants were verified for peroxisome-restoring activity in ZPG208 and intracellular localization in wild-type CHO-K1. (A) ZPG208 was transfected with cDNAs encoding fused proteins encompassing Pex3p with amino acid residues of the full-length, 1–372 (a and e), 16–372 (b and f), 1–312 (c and g), and 1–40 (d and h). Peroxisome-forming activity was verified by import of Pex3p-EGFP, in which peroxisomes were assessed by immunostaining of PTS1. Bar, 20 μm. (B) The same set of the full-length and truncated variants of Pex3p-EGFP fusion protein were also expressed in CHO-K1; peroxisomal localization of Pex3p-EGFP assessed by GFP fluorescence was confirmed by immunostaining of PTS1. Bar, 20 μm.