Table 2.
Properties of wild-type CHO-K1, ZPG208, and RnPEX3-transformed ZPG208 (208P3) cells
| Cell | Peroxisome | Total catalase activity (mU/106 cells) | Catalase latency (%) | P9OH/UV (%) | P12/UV (%) |
|---|---|---|---|---|---|
| CHO-K1 | + | 5.8 | 61 | 0 | 76 |
| ZPG208 | − | 8.0 | 5 | 81 | 0 |
| 208P3 | + | 8.2 | 55 | 0 | 72 |
Catalase latency represents peroxisomal catalase, calculated from Figure 3A, as described (Tsukamoto et al., 1990). For determination of P12/UV or P9OH/UV resistance, 200 or 1 × 105 cells were inoculated into 60-mm dishes and selected (Shimozawa et al., 1992). The numbers of colonies were counted in triplicate experiments and expressed as percentages of that of unselected control.