Table 4.
Peroxin activity and intracellular localization of Pex3p variants
| Flag-Pex3p-EGFP residues | Peroxisome-restoring activity | Localization |
|---|---|---|
| Full-length, 1–372 | + | Peroxisome |
| 16–372 | − | Cytosol |
| 31–372 | − | Cytosol |
| 110–372 | − | Cytosol |
| 151–372 | − | Cytosol |
| 1–312 | − | Peroxisome |
| 1–203 | − | Peroxisome |
| 1–40 | − | Peroxisome |
N-terminally flag tagged and C-terminally EGFP-fused full-length Pex3p and truncated variants were verified for peroxisome-restoring activity in ZPG208 and intracellular localization in wild-type CHO-K1 cells, as in Figure 9. +, complemented; −, not complemented.