Figure 2.

ABA Accumulation in Wild-Type and era3 Plants.

(A) Endogenous ABA concentrations in wild-type (WT), era3-1, and era1-2 plants. Wild-type and era3-1 values are the average of three independent measurements. era1-2 ABA content was determined only once. Averages and standard deviation are shown for WT and era3-1 mutants. FW, fresh weight.

(B) RNA transcript accumulation of zeaxanthin epoxidase (ABA1). After hybridization with the ABA1 probe, blots were stripped for β-tubulin (β-TUB) hybridization, which meant that absolute amounts could not be compared between hybridization experiments. Five micrograms of total RNA was loaded per lane.