Recovery of CARC after a Preceding Endocytosis.
In one protoplast, [Ca2+]cyt was increased in ramplike fashion (first stimulation, on the left), resulting initially in an increase and subsequently in a decrease in membrane capacitance. After 129 sec, [Ca2+]cyt was increased a second time (second stimulation on the right). This second stimulation caused a CARC with an amplitude approximately one-third that of the first one.