Figure 5.

Structures of Wild-Type and Mutant KOR1 Proteins.

A schematic diagram of the structural features of KOR1 (top) and DNA and amino acid sequences of wild-type and mutant KOR1 (bottom). Mutated residues are shown in boldface. Numbers refer to the positions of the amino acid residues in the protein. Underlined sequences indicate restriction enzyme sites introduced when the mutants were generated. GCCGGC, NaeI; GTCGAC, SalI; CSM, cellulase signature motif; PTS, polarized targeting signal; TMD, transmembrane domain; WT, wild type.