Figure 4.

Dissection of the HIR in Gain-of-Function Experiments.

(A) Schematic representation of the constructs used in the present set of experiments. X represents the variable 5′ ends, as indicated in (B) and (C); the ellipse corresponds to the 35S minimal promoters, which are −90 in (B) and −46 in (C).

(B) and (C) The −176 to −31 but not the −134 to −31 fragment of the Lhcb1*2 promoter confers HIR to a minimum 35S CaMV promoter. Seedlings transformed with the constructs described in (A) were exposed to pulses of FR light, pulses of R light, or continuous FR light. Data are means ±se of five to seven independently transformed lines. For each line, activity was normalized to the values observed after treatment with R light pulses.