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Inducible expression of p27kip1 in BALB/c-3T3-derived cell lines. (A) p27-30 and p27-47 cells were grown to 20–30% confluency and treated with 1 mM IPTG for 18 h. (B) Uninduced parental BALB/c-3T3 cells and p27-27 cells were compared for p27kip1 expression. (C) p27-47 cells received the indicated concentrations of IPTG for 24 h. (D) p27-47 cells were exposed to 1 mM IPTG for the indicated times. (A–D) Cell extracts (40 μg) were immunoblotted with antibody to p27kip1.
