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. 2000 Sep;12(9):1647–1666. doi: 10.1105/tpc.12.9.1647

Figure 2.

Figure 2.

Analysis of Early Nodulin Gene Expression in Wild-Type and Mutant Plants in Response to Nod Factors.

(A) RT-PCR analysis of MtENOD11 expression in wild type (WT) and mutant (B85, locus 4, and TR25, locus 2) roots treated with Nod factors at 10–8 M and harvested at the indicated times (see Methods). MtPR10-1 RNA was amplified as a control for the quality and quantity of the RNA samples.

(B) RNA gel blot of rip1 expression in wild type (WT) and mutant (B85, locus 4, and B129, locus 1) roots treated with S. meliloti Nod factors at 10–8 M and harvested at the indicated times (see Methods). rRNA concentrations are shown to control for equal loading.