Table 1.
Bacterial Strains, Plasmids, and Plants Used in This Studya
| Designation | Relevant Characteristics | Reference/Source |
|---|---|---|
| S. meliloti | ||
| ABS7 | Nod+Fix+ on M. truncatula | Bekki et al. (1987) |
| GMI6526 | 2011(pXLGD4), Nod+Fix+ on M. truncatula | Ardourel et al. (1994) |
| GMI6390 | 2011(pMH682) | Roche et al. (1991b) |
| GMI3198 | 2011(pGMI3194)(pXLGD4) | This study |
| GMI6628 | 2011Δ(nodF)13nodL::Tn5, PmRa | Ardourel et al. (1994) |
| GMI6630 | 2011Δ(nodF)13nodL::Tn5(pXLGD4) | Ardourel et al. (1994) |
| GMI3125 | 2011Δ(nodF)13nodL::Tn5nodC::Sp | This study |
| GMI6702 | 2011nodA::Tn5#2208(pXLGD4) | Debellé et al. (1986) |
| Plasmids | ||
| pXLGD4 | pGD499 prime (IncP) carrying a hemA::lacZ fusion, TcR | Leong et al. (1985) |
| pMH682 | pWB85a prime (IncP) carrying nodD3 and syrM of S. meliloti, TcR | Honma et al. (1990) |
| pGMI1394 | pML132 prime (IncQ) carrying nodD1 of S. meliloti, GmR | Demont et al. (1994) |
| pRmM57 | pRMSL26 (IncP) nodC::Sp, TcR | Mulligan and Long (1985) |
| pR751-pMG2 | IncP plasmid, Tra+, GmR | Jacoby et al. (1976) |
| pRK2013 | Helper plasmid for mobilization of IncP and IncQ plasmids, KmR | Ditta et al. (1980) |
| M. truncatula | ||
| Jemalong A17 | Wild type, Nod+Fix+ with S. meliloti | Penmetsa and Cook (1997) |
| Jemalong (MtENOD11–GUS) | Transgenic wild type carrying a fusion between the promoter of MtENOD11 and the β-glucuronidase (GUS) gene | D.G. Barker, J.-L. Pingret, M. Chabaud, and E.-P. Journet, unpublished results |
| TR25 | γ-ray Nod− mutant of Jemalong | Sagan et al. (1995) |
| TR26 | γ-ray Nod− mutant of Jemalong | Sagan et al. (1995) |
| TRV25 | γ-ray Nod− mutant of Jemalong | Sagan et al. (1998) |
| B85 | EMS Nod− mutant of Jemalong | R.V. Penmetsa and D. Cook, unpublished results |
| B129 | EMS Nod− mutant of Jemalong | R.V. Penmetsa and D. Cook, unpublished results |
| C54 | EMS Nod− mutant of Jemalong | R.V. Penmetsa and D. Cook, unpublished results |
| C71 | “domi,” EMS Nod− mutant of Jemalong | Penmetsa and Cook (1997) |
| P1 | EMS Nod− mutant of Jemalong | This study |
| Y6 | EMS Nod− mutant of Jemalong | This study |
a
R, resistant.