Table 2.
Genetic Analysis of Nod− Mutants
| F1b
|
F2b,c
|
||||
|---|---|---|---|---|---|
| Mutantsa | Nod+ | Nod− | Nod+ | Nod− | χ2d |
| B85 | 16 | 0 | 76 | 24 | 0.02 |
| B129 | 6 | 0 | 134 | 43 | 0.05 |
| C54 | 5 | 0 | 74 | 21 | 0.01 |
| P1 | 6 | 0 | 86 | 28 | 0.01 |
| Y6 | 2 | 0 | 73 | 27 | 0.21 |
a
Mutants were crossed to Jemalong (MtENOD11–GUS) Nod+ plants, which were used in place of wild-type plants to provide a marker for crosses (see Methods).
b
Nodulation was scored in plants of the F1 and F2 generations, 3 weeks after inoculation with S. meliloti GMI6526; numbers indicate the number of plants found to be Nod+ or Nod−. In most cases, F1 results presented are from two or more independent crosses.
c
F2 results are given for a single F2 population but were confirmed in each case by analyzing at least one additional, independent F2 population derived from a different F1 plant.
d
χ2 calculated for a 3:1 ratio of Nod+/Nod− phenotype; P > 0.05 when χ2 < 3.84.