Table 4.
Effect of Sulfated and Nonsulfated S. meliloti Nod Factors at Different Concentrations on Root Hair Deformation of Wild Type and Mutants
| Nod Factor Concentration
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sulfated
|
Nonsulfated
|
||||||||
| Plant | Phenotypea | 10−7 M | 10−8 M | 10−9 M | 10−10 M | 10−11 M | 10−12 M | 10−7 M | 10−9 M |
| Wild type | Hab | +b | + | + | + | + | − | − | − |
| B129 | Has | + | + | + | + | + | − | − | − |
| B85 | Hab | NDc | + | + | + | − | − | − | − |
| C54 | Hab | ND | + | + | + | + | − | − | − |
a
Hab indicates root hair branching; Has indicates root hair swelling.
b
Samples were scored for the percentage of plants showing deformed root hairs and then compared with control samples. (+) indicates a significantly (P = 0.01) higher percentage of responding plants; and (−) indicates no significant difference (P = 0.01) compared with control plants. At least two independent experiments were performed on 10 to 15 plants/sample, all of which were scored double blindly 16 hr after Nod factor treatment. Analysis of variance was conducted with Fisher's Exact test (Kendall and Stuart, 1976).
c
ND, not determined.