Table 1.
Use of an Adhesion Assay to Isolate the Large Molecule from the Lily Style
| Fractions | No. of Adhered Pollen Tubesa |
|---|---|
| SCA | 6 ± 3 (7) |
| Citrate soluble | 8 ± 2 (4) |
| Citrate soluble + SCA | 184 ± 34 (4) |
| CDTA soluble | 15 ± 6 (3) |
| CDTA soluble + SCA | 105 ± 41 (7) |
| Imidazole soluble | 1 ± 1 (4) |
| Imidazole soluble + SCA | 227 ± 52 (6) |
| Na2CO3 soluble | 0 (3) |
| Na2CO3 soluble + SCA | 112 ± 23 (3) |
| KOH solubleb | 0 (3) |
| KOH soluble + SCA | 35 ± 21 (3) |
| KOH insoluble | 1 ± 1 (3) |
| KOH insoluble + SCA | 23 ± 11 (3) |
a
The number of pollen tubes adhered to nitrocellulose membrane coated with 75 μg of the indicated style fraction or 75 μg of the indicated style fraction combined with 10 μg of SCA partially purified from SCA(SG). The numbers in parentheses indicate the number of replicates.
b
The five KOH extracts were combined in equal amounts (15 μg of each extract).