Table 3.
Chemical Composition of the Stylar Adhesive Fractions from the Imidazole Extraction
| Yield Recovery and Composition |
Crudea | Ethanol 40 to 60% Insoluble |
Q Sepharose Elution 300 to 400 mM NaCl |
Size Exclusion Fraction C |
|---|---|---|---|---|
| Yield (mg dry weight) | 644 | 325 | 88 | 7 |
| Total protein % (w/w) | 3.5 | 3.3 | 0.8 | 0.5 |
| Total carbohydrate % (w/w) | 86.8 | 94.6 | 96.1 | 98.6 |
| Glycosyl residue (mol%) | ||||
| Arabinosyl | 10.5 | 10.1 | 7.9 | 4.6 |
| Rhamnosyl | 4.9 | 3.9 | 5.1 | 6.6 |
| Fucosyl | 0.5 | 0.2 | 0.3 | 0.8 |
| Xylosyl | 3.9 | 1.2 | 0.9 | 1.5 |
| Mannosyl | 6.8 | 0.5 | 0.3 | 0.8 |
| Galactosyl | 12.4 | 13.8 | 10.3 | 4 |
| Glucosyl | 6.7 | 1 | 0.8 | 0.8 |
| Glucuronosyl | 13.7 | 6.4 | 5.9 | 7.6 |
| Galacturonosyl | 40.9 | 63 | 68.5 | 73.3 |
a
Crude imidazole extract was fractionated by an ethanol precipitation series (20% increment), anion-exchange chromatography (Q Sepharose eluted with NaCl series of 100 mM increment), and size-exclusion chromatography (Sepharose CL-4B).