Table 4.
Adhesion of Lily Pollen Tubes on a Matrix Coated with Active Fractions from the Imidazole Extraction and SCA after Their Treatments with Enzymes
| Fraction and Enzymatic Treatment | No. of Adhered Pollen Tubesa |
|---|---|
| Proteinase K treatmentb | |
| Imidazole extractc + SCA(SG) | 236 ± 86 (8) |
| + SCA(SG) (pK treated)d | 1 ± 1 (2) |
| + SCA(SG) (boiled pK) | 195 ± 43 (2) |
| Imidazole extract (pK treated) + SCA(SG) | 215 ± 34 (2) |
| (pK treated) + SCA(SG) (pK treated) | 13 ± 8 (2) |
| PG2 treatmente | |
| Fraction Cf + SCA(SE) | 401 ± 53 (5) |
| Fraction C (PG2 treated)g + SCA(SE) | 51 ± 16 (3) |
| Fraction C (boiled PG2) + SCA(SE) | 311 ± 43 (2) |
Numbers in parentheses indicate the number of replicates.
Adhesion assay using 75 μg of imidazole extract combined with 10 μg of SCA partially purified from SCA(SG).
Crude imidazole extract precipitated between 40 and 60% ethanol.
Imidazole extract and SCA were separately incubated with proteinase K (pK treated) or boiled proteinase K (boiled pK) and combined for the adhesion assay.
Adhesion assay using 50 μg of imidazole extract (Fraction C) combined with 5 μg of SCA isolated from SCA(SE).
Fraction C of the size-exclusion column as identified in Figure 1.
Fraction C was incubated with PG2 or with boiled PG2 and combined with SCA. The ratio of the amount of reducing sugars at the end and at the start of the PG2 treatment was 9. In the same conditions, the ratio obtained with the commercial polygalacturonic acid was 40.