Table 5.
Adhesion Assays Using Various Commercial Polymers to Substitute for the Lily Stylar Pectin (Fraction C) or SCA
| Fractions | No. of Adhered Pollen Tubesa |
|---|---|
| Fraction C + SCA(SE) | 401 ± 53 (5) |
| BSA + SCA(SG) | 0 (8) |
| Alginic acid + SCA(SE) | 0 (4) |
| Gum arabic + SCA(SG) | 5 ± 2 (6) |
| Dextran + SCA(SE) | 0 (4) |
| PGAb + SCA(SE) | 105 ± 34 (5) |
| PEc + SCA(SE) | 15 ± 3 (4) |
| Fraction C + cytochrome c | 10 ± 5 (9) |
| Fraction C + poly-d-lysine | 9 ± 2 (4) |
a
Number of pollen tubes adhered to nitrocellulose membrane coated with 50 to 200 μg of the fractions combined with 5 to 20 μg of SCA partially purified from SCA(SG) or isolated from SCA(SE) or with 5 to 100 μg of cytochrome c or poly-d-lysine. The numbers in parentheses indicate the number of replicates.
b
Polygalacturonic acid.
c
Pectin esterified with a degree of esterification of 93%.