Figure 7.

Quantification of Incorporation of Newly Synthesized D1 Protein into PSII Subcomplexes.

Accumulation of radioactivity in the precursor and mature D1 protein in different PSII subpopulations was quantified after 5- and 20-min chases. Each chase was conducted with no additional chemicals (Control) or in the presence of DTTred (DTT), NEM, or IBZ, and PSII subcomplexes were separated, as given in the legend to Figure 6. Relative amounts of radioactivity in unassembled D1 protein (free D1 protein), PSII reaction centers, CP43-less PSII monomers, and intact PSII monomers (including CP43) are indicated. The data are expressed as the percentage of accumulated total labeled precursor and mature D1 protein.