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. 2000 Sep;12(9):1783–1794. doi: 10.1105/tpc.12.9.1783

Figure 1.

Figure 1.

Antagonism of Peptide flg15-Δ7 to Alkalinization Response of Suspension-Cultured Tomato Cells Treated with Agonist flg15.

(A) Extracellular alkalinization in suspension-cultured tomato cells treated with 0.3 nM flg15 (RINSAKDDAAGLQIA) alone (control) or in combination with 10 μM flg15-Δ7 (RINSAKDD) added at the times indicated (open circles). Extracellular pH of untreated cells was 5.1.

(B) Alkalinization response of cells treated with combinations of 0.3 nM flg15 and either 1 μM flg15-Δ7 (closed circles) or 10 μM flg15-Δ7 (open inverted triangles). The x axis indicates the time of flg15-Δ7 addition with respect to the addition of flg15. Thus, negative values indicate a pretreatment with flg15-Δ7, and positive values indicate addition of flg15-Δ7 after flg15. Control cells treated with 0.3 nM flg15 alone showed an alkalinization response with a mean ΔpHmax of 0.86 (sd = 0.03, n = 4 replicates). The time dependence of inhibition by flg15-Δ7 was reproduced in three independent series of experiments with different batches of cells.