Figure 8.

GFP-clathrin localization during Con A capping. Clathrin-minus cells expressing GFP-clathrin were treated with Con A labeled with Texas Red and imaged 2 (A and B), 4.5 (C and D), and 6 (E and F) min after Con A treatment for GFP (A, C, and E) and Texas Red (B, D, and F). Initially GFP-clathrin did not colocalize with the C-shaped Texas Red cap formed early in capping (C and D), but once the cap coalesced into a compact spot, GFP-clathrin colocalized with the Texas Red-Con A cap (E, arrows).