Figure 4.

Protein Gel Blot Analysis of the DS9 Protein in Tobacco Leaf Tissue.

Total protein extracts (lane 1), a stroma fraction (lane 2), and a thylakoid (Thylak.) fraction (lane 3) prepared from healthy leaves of Samsun NN tobacco plants were subjected to protein gel blot analysis with antibodies raised against DS9ΔN. Lanes 1, 2, and 3 contain total protein extracts containing 20 μg of total protein, the stroma fraction equivalent to the volume of thylakoid membrane fraction used, and the thylakoid membrane fraction containing 5 μg of chlorophyll, respectively. The arrowhead indicates the relative molecular masses of ∼78 kD. Size markers are indicated at left in kilodaltons.