Figure 6.

Protein Gel Blot Analysis of the DS9 Protein in TMV- and Mock-Inoculated NN Tobacco Leaves after the Temperature Shift.

(A) Detached healthy leaves of Samsun NN tobacco plants were inoculated with TMV (10 μg/mL) or buffer only (Mock) and shifted from 30 to 20°C at time 0. Leaves were harvested at the indicated times after the temperature shift and used for protein extraction. Twenty micrograms of total protein was immunoblotted with antibodies raised against DS9ΔN (Ab-DS9). The arrowhead indicates the relative molecular masses of ∼78 kD. The blots were reprobed with antibodies (Ab-SIPK) against a synthetic peptide corresponding to the N-terminal 24–amino acid sequence of SIPK as a loading control.

(B) Relative amount of the DS9 protein in TMV-inoculated leaves as shown in (A), expressed as a ratio with that present in the control sample (0 hr after the temperature shift), which was set equal to 100%. Values are the mean ±sd from three independent experiments.