Figure 5.

Injection of Antibody during Anaphase.

(A) Injection with KCBP-Ab did not affect anaphase; the chromatids separated normally (0 to 15 min after injection). At the end of anaphase, no clear zone or initiation of the cell plate was visible (20 min). The whole spindle apparatus tilted 45° (30 min), and the cell was arrested in telophase for >2 hr.

(B) The cell was injected with preimmune serum and progressed through anaphase and telophase normally. At the end of anaphase (20 min), a phragmoplast formed and vesicles coalesced to form a cell plate. The new cell wall was formed in ∼30 min (49 min after injection).

The time in minutes after injection is shown at the bottom right of each photograph. .