Figure 9.

Representative Mobility Shift Assays of Cosynthesis Reaction Mixtures of Human and Arabidopsis Polypeptides.

Cosynthesis mixtures were incubated with ³²P-labeled homoduplex DNA (T/A, lane 1, all panels), or heteroduplex DNA (T/G, C/C, +1 IDL [insertion–deletion DNA with one T nucleotide looped out], and +3 IDL [insertion–deletion DNA with AAG nucleotides looped out], lanes 2 to 5, respectively, all panels) and analyzed on nondenaturing PAGE (see Methods).

(A) Representative mobility shift assay of hMSH2•hMSH6 cosynthesis mixtures.

(B) Representative mobility shift assay of AtMSH2•AtMSH6 cosynthesis mixtures.

(C) Representative mobility shift assay of AtMSH2•AtMSH7 cosynthesis mixtures.

(D) Representative mobility shift assay of AtMSH2•AtMSH3 cosynthesis mixtures.