Figure 3.

NEM and EDTA increase the amount of kinesin in membrane fractions after subcellular fractionation. (a) Bar graphs showing partitioning of total protein and kinesin in cytosolic versus membrane fractions. Changes in the partitioning of kinesin in the absence (control) or presence of NEM, EDTA, or NEM and EDTA combined are shown. Average kinesin levels were calculated from results of two quantitative immunoblots. Bars indicate the range of variation. (b) Representative immunoblots showing the partitioning of kinesin, hsc70, and the integral membrane protein p38 in control and treated samples. The partitioning of total protein, hsc70, and p38 between cytosolic and membrane fractions was unaltered by the various treatments. However, all treatments dramatically shifted kinesin from cytosolic to membrane fractions.