Figure 7.

Relocation of nucleolar molecules in NOY 558 according to rDNA transcription state. After inhibition of rRNA synthesis in NOY 558 (4 h on glucose), plasmid pNOY 103 was detected both in the mininucleoli (arrow) and in the nucleoplasm (A). The nuclear labeling corresponding to rRNA was weaker than in rDNA-transcribing cells (Figure 2H) and was preferentially found in the mininucleoli (B, arrows). The upstream region of the ETS 1 of 35S rRNA was localized by ISH. The residual component (arrow) was not significantly labeled (C). Nop1p was visible in the residual component (arrow) but was also dispersed throughout the nucleoplasm (D). After resumption of rDNA transcription, plasmids (E), rRNA (F), ETS 1 (G), and Nop1p (H) were preferentially found in the peripheral component, as before inhibition (arrows). Bar, 300 nm.